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IN Cell Analyzer 1000InCell 1000 GE lifesciences High-content screening unit. InCell 1000 summary IN Cell Analyzer 1000 is a flexible, modular, cellular and subcellular imaging system for fast, automated imaging in fixed and live cells. This unit is an automated epifluorescence-based microscope with motorised stage, laser autofocus and image acquisition software. It uses a Nikon microscope and optics with a xenon light source and a range of Chroma excitation/emission filters as well as Chroma multi-pass dichroics. The unit in the DDBL also has temperature control, automated liquid handling, a transmitted light unit, microscope slide imaging and an optical Z-sectioning module. This unit is primarily designed for high-content, high throughput assays. It is well suited to image capture from multi well plates (up to 96 well). Although it does provide excellent epiflourescent image quality it is not designed primarily for capturing publication quality images or for quickly looking at samples to assess staining. These tasks are better done on microscopes designed for this purpose. Using the InCell 1000 Monash users outside the DDBL may use the InCell 1000, however you will need to be trained and registered. You will need to be trained on use of both the InCell 1000 itself as well as the accompanying In Cell investigator software. The InCell 1000 is located in building 13E (Pharmacology). If you would like to arrange training for the InCell 1000 you will need to contact either Carline Hick: caroline.hick@med.monash.edu.au Registered users will be able to book time on the InCell 1000 using either Netscape calendar or the Oracle client. Charges: Offline data analysis No charge but by negotiation (see below) InCell 1000 objectives InCell 1000 filters and dichroics Filters are listed with transmission maxima followed by transmission bandwidth. Excitation Emission Example dye Note that the excitation and emission filters can be selected independently, therefore it is possible to accommodate conjugated dyes and FRET style experiments.
Dichroic Example Dyes Link InCell software support In Cell investigator software is available for data captured on the InCell 1000. Presently we have 2 licences for this software. Data sets acquired on the InCell 1000 are typically very large and can take many hours for the software to analyse. Users will therefore have to negotiate with Caroline or Sebastian for use of computer time to perform data analysis or alternatively purchase their own copy of the s |