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BD FACSCanto II
The BD FACSCanto II analyser
is equipped with 3 lasers, 2 light scatter detectors and 8 fluorescence
detectors. The
instrument is easy to startup and does not require daily laser
alignment. Samples can be run on this instrument at a maxiumum of
10,000 cells/second.
In the table below are some of the common fluorochromes that can be used in conjunction on this instrument:
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FACSCanto II analyser
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| Laser | Detector | Filters | Common Fluorochromes | 488nm
| FL1 | 530/30 | FITC
Alexa Fluor 488
EGFP
CFSE | | FL2 | 585/42 | PE | | FL3 | 670LP | Pe-Cy5
PE-Cy5.5
PI
PerCP
PerCP-Cy5.5
Qdot705
| | FL4 | 780/60 | PE-Cy7
| | 635nm | FL5 | 660/20 | APC
Alexa Fluor 647 | | FL6 | 780/60 | APC-Cy7
APC-Alexa Fluor 750 | | 405nm | FL7 | 450/50 | Pacific Blue
| | FL8 | 510/50 | AmCyan
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