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Beckman Coulter MoFlo BTA
The Beckman Coulter MoFlo BTA is a high speed cell analyser.
It is equipped with 2 lasers, 2 light scatter detectors and 5
fluorescence detectors. The instrument uses a 100�m nozzle and
runs at a pressure of 35psi. With this setup, it is capable of
running samples at speeds of up to 15,000 cells/second.
In the table below are some of the common fluorochromes that can be used in conjunction on this instrument:
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MoFlo BTA analyser
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| Laser | Detector | Filters | Common Fluorochromes | 488nm
| FL1 | 530/40 | FITC
Alexa Fluor 488
EGFP
CFSE | | FL2 | 570/70 | PE | | FL3 | 670/40 or
700/30 | PE-Cy5
PE-Cy5.5
PI
Qdot 655
Qdot 705 | | 633nm | FL4 | 740LP | APC-Cy7
APC-AlexaFluor 750
| | FL5 | 670/40 | APC
Alexa Fluor 647 |
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