Telephone: +61 - 3 9902 9226
Research in the Wilce lab focuses on the study of protein-peptide and protein-oligonucleotide interactions that occur in human cells to control gene expression, cell growth and cell proliferation.
The laboratory, run jointly with Prof Matthew Wilce, aims to better understand molecular recognition by determining the overall shapes, as well as the precise three-dimensional structures of macromolecular complexes, and by testing their affinity and specificity using biophysical techniques. We investigate several molecular systems involved in cell inflammation and cancer cell migration. These include the study of proteins which bind to mRNA and regulate translation (in collaboration with Dr Myriam Gorospe, NIH), proteins which recognise RNA in innate immunity (in collaboration with Professor Bryan Williams, MIMR) and the development of peptides which inhibit cancer cell proliferation and migration (in collaboration with Professor Krag and Dr Stephanie Pero, USA).
About Jackie Wilce
I completed a B. Sc (Hons) degree at Monash University, specialising in novel peptide chemistries at honours level. In 1994 I graduated with a Ph.D. in Medicinal Chemistry from the Victorian College of Pharmacy (now MIPS, Monash University) in the field of NMR structural and dynamics studies of peptides and proteins. I then took up a position at Queensland University in the Centre for Drug Design and Development with Professor David Craik, synthesising proteins using peptide and novel linkage chemistries. I then spent several years at the University of Sydney with Professor Glenn King, extending my expertise to include molecular biological and protein expression and purification techniques as well as 3D heteronuclear NMR spectroscopy and analytical ultracentrifugation. Here I began to develop an interest in structural and dynamic studies of novel cancer targets as well as the use of peptides for transporting bioactive compounds across cell walls. I also embarked on structural studies of a protein:DNA complex involved in replication fork arrest for which I was awarded an ARC Postdoctoral Fellowship. In 1999 I established my own laboratory as a joint member of Biochemistry and Chemistry at the Department at the University of Western Australia, where I extended my research into the field of structural and dynamic studies of protein-RNA interactions involved in mRNA stabilization in collaboration with partner, Prof Matthew Wilce. In 2001 I was awarded an ARC Fellowship to pursue molecular interactions studies, and have added surface plasmon resonance and isothermal titration calorimetry to the repertoire of biophysical techniques that underpins the work. In 2005 I moved with Matthew Wilce to Monash University to establish labs in the dynamic Department of Biochemistry and Molecular Biology. I was appointed as a Senior lecturer in 2007 and promoted to Associate Professor in 2010. I now engage in both teaching and research in the macromolecular sciences.
There are currently positions vacant for scholarship supported students to study at Honours and PhD level under my supervision at Monash University. Prospective students should have an excellent background in biochemistry, molecular biology or chemistry, and a passion for studies of macromolecular interactions. Please email me at firstname.lastname@example.org if you are interested in studying in my laboratory.
I am engaged at all levels of undergraduate and graduate teaching. I am current convenor of the second year unit "Introduction to Bioinformatics" within the Bachelor of Biomedical Science (BMS2062) and also teach within several 3rd year Biochemistry Units (BCH3052, BCH3031 and BCH3990).
Watson GM, Gunzburg MJ, Ambaye ND, Lucas WA, Traore DA, Kulkarni K, Cergol KM,
Payne RJ, Panjikar S, Pero SC, Perlmutter P, Wilce MC and Wilce JA. Cyclic peptides
incorporating phosphotyrosine mimetics as potent and specific inhibitors of the
Grb7 breast cancer target. J Med Chem. 2015 Sep 11. [Epub ahead of print]
Gunzburg MJ, Sivakumaran A, Pendini NR, Yoon JH, Gorospe M, Wilce MC and Wilce
JA. Cooperative interplay of let-7 mimic and HuR with MYC RNA. Cell Cycle. 2015
Waris S, Wilce MC and Wilce JA. RNA recognition and stress granule formation by
TIA proteins. Int J Mol Sci. 2014 Dec 16;15(12):23377-88.
Cruz-Gallardo I, Aroca Á, Gunzburg MJ, Sivakumaran A, Yoon JH, Angulo J,
Persson C, Gorospe M, Karlsson BG, Wilce JA and Díaz-Moreno I. The binding of TIA-1
to RNA C-rich sequences is driven by its C-terminal RRM domain. RNA Biol.
Ambaye ND, Gunzburg MJ, Traore DA, Del Borgo MP, Perlmutter P, Wilce MC and Wilce
JA. Preparation of crystals for characterizing the Grb7 SH2 domain before and
after complex formation with a bicyclic peptide antagonist. Acta Crystallogr F
Struct Biol Commun. 2014 Feb;70(Pt 2):182-6.
Lim RC, Price JT and Wilce JA. Context-dependent role of Grb7 in HER2+ve and
triple-negative breast cancer cell lines. Breast Cancer Res Treat. 2014
Kim HS, Headey SJ, Yoga YM, Scanlon MJ, Gorospe M, Wilce MC and Wilce JA.
Distinct binding properties of TIAR RRMs and linker region. RNA Biol. 2013
A/Prof Jackie Wilce
Department of Biochemistry and Molecular Biology
19 Innovation Way (Building 76)
CLAYTON VIC 3800
ph: +613 9902 9226
fax: +613 9902 9500