| Staff Listings |
Dr Isabelle Lucet
Senior Research Fellow
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Tel: +61 3 990 29245
Fax: +61-3-990 29500
Office: Building 77, Room 211
Email: isabelle.lucet@monash.edu
Research Group Members
Ms Rebecca Bamert
Ms Gilu Abraham
Dr Megan Bird
Research Activities
Towards an integrated screening program for the Plasmodium falciparum kinome Project funded by Medicine for Malaria Venture (MMV).
The Lucet laboratory focuses upon understanding the structure, function and biology of Plasmodium falciparum kinases that play a fundamental role in malaria pathogenesis. We are particularly interested in developing an integrated screening program for the P. falciparum kinome together with a high-throughput crystallographic program to ultimately lead to the development of urgently needed new anti-malarial drugs.
Techniques/Expertise
Molecular biology, recombinant protein expression using insect and bacterial cells, protein purification, X-ray crystallography, enzyme assays, thermal shift assay and development of affinity-based assays.
Collaborations
Monash Institute of Pharmaceutical Sciences (MIPS) -Pr Bill Charman/ Pr Andrew Wilks/ Pr Martin Scanlon
SYNthesis (http://www.synmedchem.com)
EPFL, Switzerland - Pr Christian Doerig
SAM collaborative project: http://www.scientistsagainstmalaria.net/
GSK, Tres Cantos, Spain
Selected Publications
1. * Williams, N. K.; Bamert, R. S.; Patel, O.; Wang, C.; Walden, P. M.; Wilks, A. F.; Fantino, E.; Rossjohn, J.; Lucet, I. S. Dissecting specificity in the Janus kinases: the structures of JAK-specific inhibitors complexed to the JAK1 and JAK2 protein tyrosine kinase domains. Journal of Molecular Biology (2009) 387 1 219-32
2. * Williams, N. K.; Lucet, I. S.; Klinken, S. P.; Ingley, E.; Rossjohn, J. Crystal structures of the Lyn protein tyrosine kinase domain in its Apo- and inhibitor-bound state. Journal of Biological Chemistry (2009) 284 1 284-91
3. * Walter, M.; Lucet, I. S. (co-first author); Patel, O.; Broughton, S. E.; Bamert, R.; Williams, N. K.; Fantino, E.; Wilks, A. F.; Rossjohn, J. The 2.7 A crystal structure of the autoinhibited human c-Fms kinase domain. Journal of Molecular Biology (2007) 367 3 839-47
4. * Lucet, I. S.; Fantino, E.; Styles, M.; Bamert, R.; Patel, O.; Broughton, S. E.; Walter, M.; Burns, C. J.; Treutlein, H.; Wilks, A. F.; Rossjohn, J. The structural basis of Janus kinase 2 inhibition by a potent and specific pan-Janus kinase inhibitor. Blood (2006) 107 1 176-83
