Professor David A Jans

BSc (Hons, University of Melbourne), PhD (JCSMR, Canberra)

Present address: Prof. David A. Jans c/- Nuclear Signalling Lab. Department of Biochem. & Mol. Biol. P.O box 13D Monash University VIC 3800 Australia Telephone: +61 3 9905 3778 Facsimile: +61 3 9905 4699 email: David.Jans@med.monash.edu.au

General Profile:

David Andrew Jans is originally from Melbourne. After graduating (BSc. Hons) from the University of Melbourne Microbiology Dept. in 1980, he joined the Dept. of Biochemistry at the John Curtin School of Medical Research (JCSMR) to carry out his Ph.D. studies with Graeme Cox and Frank Gibson on bacterial ATPase (completed 1984). He then took up a research scientist position at the Friedrich Miescher Institut in Basel (Switzerland), followed by a visiting fellowship at the Max Planck Institut für Biophysik in Frankfurt am Main (Germany), working in the area of phosphorylation and signal transduction in mammalian cells. In 1990, he became a Senior Scientist at the Institut für Medizinische Physik und Biophysik, Westfälische Wilhelms-Universität in Münster (Germany), turning his focus to the use of microscopic techniques to investigate transport processes. He returned in 1993 to the JCSMR (Division of Biochem. & Mol. Biol.), initially as a Fellow, to establish the Nuclear Signalling Laboratory, with a strong focus on the processes of protein transport into the nucleus. He rose to Senior Fellow in 1998, Full Professor in 2000, and since 1998 also holds a conjoint teaching appointment (Associate Professor/Professor) at the James Cook University of North Queensland (Townsville, Australia).

He has received a number of awards (13 during his University undergraduate and Ph.D studies), and has been awarded 23 grants since 1994 (c. $ 2.6 million AUS.) including awards from the Australian Research Council (International Research Fellowship, ARC-DEETYA SPIRT grant and Project Grant, and RIEF scheme), Clive and Vera Ramaciotti Foundation, UICC (International Union against Cancer - Biotechnology Transfer), DIST (Dept. of Industry, Science and Technology - Bilateral Science and Technology Program), NHMRC (National Health and Medical Research Council - CARG Grant), the Rebecca L. Cooper Medical Research Foundation Ltd., and Wellcome Foundation He has also been Associate/Co-Investigator on various other successful grants, including 3 NHMRC Research Project Grants, a $1,000,000 Australian Cancer Research Foundation grant, and two Wellcome equipment grants. In collaboration with Prof. Sobolev and his group at Moscow State University (Russia) he is coauthor of a patent ("A Composition to Effect Photodynamic Damage of Target Cells and a Method to Effect Photodynamic Damage of Target Cells" which won a Medaille d'Or avec Mention at the 45th. World Salon of Inventions - "Brussels - Eureka 96"). He won the IRPC (International Research Progress Council) Eminent Scientist of the Year Award for Molecular Biology in 1999. He has been invited to speak at 10 international meetings in the last 6 years, and has been invited to submit 10 review articles to prestigious journals, as well as a monograph. He has 132 full publications in all (117 in refereed journals such as EMBO J., J. Cell Biol., Proc. Natl. Acad. Sci. USA, Mol. Cell. Biol., and J. Biol. Chem.) in the fields of bioenergetics, signal transduction, biochemical and genetic analysis of membrane receptors, phosphorylation, nuclear protein transport, photodynamic and gene therapy, as well as a monograph ("The mobile receptor hypothesis: the role of membrane receptor lateral movement in signal transduction") and 72 published abstracts.

Research Links:

Nuclear Signalling

FULL PUBLICATION LIST

Monograph

M1. Jans, D.A. The mobile receptor hypothesis: the role of membrane receptor lateral movement in signal transduction. R.G. Landes Bioscience Publishers, Austin, Texas. 1997. 224 pages.

Book Chapters

B1. Cox, G.B., Jans, D.A., Fimmel, A.L., Gibson, F. & Hatch, L. (1984) "The role of the b- subunit in the integrated assembly of the F1F0-ATPase" in H+-ATP Synthase: Structure, Function and Biogenesis of the F1F0 Complex of Coupling Membranes (Papa, S., Altendorf, K., Ernster, L. & Packer, L., eds.) pp 89-97, ICSU Press/Adriatica Editrice, Bari, Italy

B2. Fahrenholz, F., Kojro, E. & Jans, D.A. (1988) "Renal and hepatic vasopressin receptor proteins: identification and strategies for purification" in Vasopressin: Cellular and Integrative Functions (Cowley, A.W.Jnr., Liard, J.F. & Ausiello, D.A., eds.) pp 27-32, Raven Press, NY

B3. Fahrenholz, F., Jurzak, M., Pavo, I., Kojro, E., Hackenberg, M., Zsigo, J. & Jans, D.A. (1989) "Molecular properties of receptors for neurophyseal hormones" in Peptides 1988 (Jung, G. & Bayer, E., eds.), p 534-536, Walter de Gruyter, Berlin, NY

B4. Fahrenholz, F., Luzius, H. & Jans, D.A. (1989) "Irreversible V2-receptor activation: effect on down-regulation of the cAMP-mediated cellular response" Colloques INSERM: Hormones and Cell Regulation 198, 21-26

B5. Fahrenholz, F., Jans, D.A. & Peters, R. (1991) "Lateral mobility of the V1- and V2- receptors in plasma membranes: a role in signal transduction and receptor down-regulation" Colloques INSERM: Vasopressin 208, 49-56

B6. Sobolev, A.S., Rosenkranz, A.A., Yachmenev, S.V., Serebriakova, N.V., Gulak, P.V., Akhlynina, T.V., Nikitin, V.A, Kiseleva, T.G., Bubchikov, V.I., Jans D.A. & Peters, R. (1992) "Creation of a molecular construct for targeted delivery of genetic material into cells" In Genetic and Cellular Engineering (All-Union Congress for Future Directions) II, 52-53 (in Russian) Puschino

B7. Rihs, H-P., Rozynek, P., Jans, D.A., Hankeln, T. & Baur, X. (1993) "Construction, expression and purification of fusion proteins that carry an allergenic sequence functioning as T- and B-cell epitope" in Molecular Biology and Immunology of Allergens (Kraft, D. & Sehon, A., eds.), pp 297-298, CRC-Press Inc.

B8. Sobolev, A.S., Rosenkranz, A.A., Akhlynina, T.V., Murav'ev, V.I., Nikitin, V.A, Gulak, P.V., Kiseleva, T.G., Turchinsky, M.F., Jans, D.A. & Vetzker, R. (1993) "Creation of molecular constructs for the targeted delivery of genetic material into cells" In Genetic and Cellular Engineering (All-Russian Congress for Future Directions) III, 40 (in Russian) Puschino, Moscow

B9. Sobolev, A.S., Rosenkranz, A.A., Jans, D.A., Akhlynina, T.V. & Murav'ev, V.I. (1993) "Receptor-mediated endocytosis and nuclear transport of artifical constructs carrying DNA or phototoxins" 13th European Workshop on the Cell Nucleus (Hidvegi, E.J., ed.) p. 115, Balatonaliga, Budapest

B10. Sobolev A. S., Rosenkranz A. A., Akhlynina T. V., Smirnova O. A., Statsiuk N. V., Naroditsky B. S., Jans P. and Jans D. A. (1995) "Nuclear localisation signals and adenoviruses enhance the uptake and nuclear transport of internalizible chlorin e6 carrying constructs" 14th International Workshop on the Cell Nucleus (The Wilhelm Bernhard workshop) p. 47, "Sol Cress", SPA, Belgium.

B11. Jans, D.A. (1996) "Protein transport to the nucleus and its regulation" in OUP Frontiers in Molecular Biology: Protein Targeting (Hurtley, S.M., ed.), Science International, ICRL
Press, pp 25-62

B12. Trapani, J.A., Jans, D.A. & Sutton, V. (1998) "Lymphocyte granule-mediated cell death" in Apoptosis and killer cell function (Vaux, D.A., ed.), Springer Seminars in Immunopathology, Geneva, 19, 323-343

B13. Trapani, J.A. & Jans, D.A. (1999) "Lymphocyte-mediated cytolysis: dual apoptotic pathways with overlapping cytoplasmic and nuclear signalling pathways" in Apoptosis: biology, mechanisms and role in disease (Kumar, S., ed.), Results Probl. Cell Differ. 23, 75-102

B14. Jans, D.A. (2000) "Nuclear protein import: methods" in Encyclopedia of Life Sciences, MacMillan Reference Ltd., Managing Editor S. Robertson, A0002616.html

B15. Jans, D.A. & Forwood, J.K. (2002) "Nuclear protein import: distinct intracellular import receptors for different types of import substrates" in "Nuclear import and export" (V. Citovsky and T. Tzvi, eds.), R.G. Landes, Bioscience Publishers, Austin, Texas, in the press; http://www.eurekah.com/reports/cellmetabolism/tzfira/10/jans10.html.

B16. Jans, D.A., Thomas, R.J. & Gillespie, M.T. (2002) "Parathyroid hormone-related protein (PTHrP): a nucleocytoplasmic shuttling protein with distinct paracrine, and intracrine roles" Vitamins and Growth Factors, in the press

Full Publications in Refereed Journals

1. Jans, D.A., Fimmel, A.L., Langman, L., James, L.B., Downie, J.A., Senior, A.E., Ash, G.R., Gibson, F. & Cox, G.B. (1983) "Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli" Biochem. J. 211, 717- 726

2. Jans, D.A. & Gibson, F. (1983) "Cloning of the structural genes of the Escherichia coli adenosine triphosphatase complex" Methods in Enzymol. 97, 176-187

3. Fimmel, A.L., Jans, D.A., Langman, L., James, L.B., Ash, G.R., Downie, J.A., Senior, A.E., Gibson, F. & Cox, G.B. (1983) "The F1F0-ATPase of Escherichia coli. Substitution of proline by leucine at position 64 in the c-subunit causes loss of oxidative phosphorylation" Biochem. J. 213 451-458

4. Cox, G.B., Jans, D.A., Gibson, F., Langman, L., Senior, A.E. & Fimmel, A.L. (1983) "Oxidative phosphorylation by mutant Escherichia coli membranes with impaired proton permeability" Biochem. J. 216, 143-150

5. Surin, B.P., Jans, D.A., Fimmel, A.L., Shaw, D.C., Cox, G.B. & Rosenberg, H. (1984) "The structural gene for the phosphate repressible phosphate binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene" J. Bacteriol. 157, 772-778

6. Jans, D.A., Fimmel, A.L., Hatch, L., Gibson, F. & Cox, G.B. (1984) "An additional acidic residue in the membrane portion of the b-subunit of the energy-transducing adenosine triphos-phatase of Escherichia coli affects both assembly and function" Biochem. J. 221, 43-51

7. Jans, D.A., Hatch, L., Fimmel, A.L., Gibson, F. & Cox, G.B. (1984) "An acidic or basic amino acid at position 26 of the b-subunit of the Escherichia coli F1F0-ATPase impairs membrane proton permeability: suppression of the uncF469 nonsense mutation" J. Bacteriol. 160, 764-770

8. Cox, G.B., Jans, D.A., Fimmel, A.L., Gibson, F. & Hatch, L. (1984) "The mechanism of ATP-Synthase: conformational change by rotation of the b-subunit" Biochim. Biophys. Acta 768, 201-208

9. Jans, D.A., Hatch, L., Fimmel, A.L., Gibson, F. & Cox, G.B. (1985) "Complementation between uncF alleles affecting assembly of the F1F0-ATPase complex of Escherichia coli" J. Bacteriol. 162, 420-426

10. Fimmel, A.L., Jans, D.A., Hatch, L:, Gibson, F. & Cox, G.B. (1985) "The F1F0-ATPase of Escherichia coli: the substitution of alanine by threonine at position 25 in the c-subunit affects function but not assembly" Biochim. Biophys. Acta 808, 252-258

11. Jans, D.A. & Hemmings, B.A. (1986) "LLC-PK1 cell mutants in cAMP metabolism respond normally to phorbol esters" FEBS Lett. 205, 127-131

12. Jans, D.A., Resink, T.J., Wilson, E-L., Reich, E., & Hemmings, B.A. (1986) "Isolation of a mutant LLC-PK1 cell line defective in hormonal responsiveness: a pleiotropic lesion affecting receptor function" Eur. J. Biochem. 160, 407-412

13. Hemmings, B.A., Schwarz, M., Adavani Rao, S. & Jans, D.A. (1986) "Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP-dependent protein kinase" FEBS Lett. 209, 219-222

14. Jans, D.A., Resink, T.J. & Hemmings, B.A. (1987) "Complementation between LLC-PK1 mutants affected in polypeptide hormone receptor function" Eur. J. Biochem. 162, 571-576

15. Botterell, S. H., Jans, D. A. & Hemmings, B. A. (1987) "Characterization of two mutants of the LLC-PK1 porcine kidney cell line affected in the catalytic subunit of the cAMP- dependent protein kinase" Eur. J. Biochem. 164, 39-44

16. Jans, D.A., Resink, T.J. & Hemmings, B.A. (1987) "Dependence of urokinase-type- plasminogen-activator induction on cAMP-dependent protein kinase activation in LLC- PK1 cells" Biochem. J. 243, 413-418

17. Nowak, I.E., Seipel, K., Jans, D.A., Schwarz, M. & Hemmings, B.A. (1987) "Characterization of a cDNA and 5' upstream of the gene encoding the Regulatory Type I subnuit of the porcine cAMP-dependent protein kinase" Eur. J. Biochem. 167, 27-33

18. Jans, D.A., Dierks-Ventling, C. & Hemmings, B.A. (1987) "Pathway of urokinase-type- plasminogen-activator induction in LLC-PK1 and T47D cells" Exper. Cell Res. 172, 76-83

19. Jans, D.A., Gajdas, E.L., Dierks-Ventling, C., Hemmings, B.A. & Fahrenholz, F. (1987) "Long term stimulation of cAMP production in LLC-PK1 cells by salmon calcitonin or a photoactivatable analogue of vasopressin" Biochim. Biophys. Acta 930, 392-400

20. Oehen, S., Parsons, J.A., Jans, D.A. & Hemmings, B.A. (1987) "Recombinant type I regulatory subunit of the cAMP-dependent protein kinase is biologically active" FEBS Lett. 220, 47-51

21. Jans, D.A., Botterell, S.H. & Hemmings, B.A. (1988) "Codominant expression of a mutation affecting cAMP-dependent protein kinase catalytic subunit in LLC-PK1 cell somatic cell hybrids" Exper. Cell Res. 176, 129-140

22. Jans, D.A. & Hemmings, B.A. (1988) "cAMP metabolism in the porcine epithelial cell line LLC-PK1: the central role of the cAMP-dependent protein kinase in cAMP-mediated gene induction" Advances in Second Messenger and Phosphoprotein Res. 21, 109-121

23. Muakkassah-Kelly, F.F., Jans, D.A., Lydon, N., Bieri, F., Waechter, F., Bentley, P. & Stäubli, W. (1988) "Electroporation of cultured adult rat hepatocytes with the c-myc gene potentiates DNA synthesis in response to epidermal growth factor" Exper. Cell Res. 178, 296-306

24. Nigg, E.A., Eppenberger, H.M., Jans, D.A., Hemmings, B.A. & Hilz, H. (1988) "Evidence for transcriptional activation of the plasminogen activator gene by the catalytic subunit of the cAMP-dependent protein kinase" UCLA Symposia on Molecular and Cellular Biology New Series 67, 169-177

25. Jans, D.A., Peters, R., Zsigo, J. & Fahrenholz, F. (1989) "The adenylate cyclase-coupled vasopressin V2-receptor is highly laterally mobile in membranes of LLC-PK1 renal epithelial cells at physiological temperature" EMBO J. 8, 2481-2488

26. Luzius, H., Jans, D.A. & Fahrenholz, F. (1989) "Use of a UV-activatable analogue of vasopressin to select mutants of LLC-PK1 cells affected in hormonal responsiveness" J. Receptor Res. 10, 61-80

27. Jans, D.A., Bergmann, L., Peters, R. & Fahrenholz, F. (1990) "Biotinyl analogues of vasopressin as biologically active probes for vasopressin receptor expression in cultured cells" J. Biol. Chem. 265, 14599-14605

28. Jans, D.A., Peters, R. & Fahrenholz, F. (1990) "Lateral mobility of the phospholipase-C- activating vasopressin V1-type receptor in A7r5 smooth muscle cells: a comparison with the adenylate cyclase-coupled V2-receptor" EMBO J. 9, 2693-2699

29. Jans, D.A., van Oost, B., Ropers, H.H. & Fahrenholz, F. (1990) "Derivatives of somatic cell hybrids which carry the human gene locus for nephrogenic diabetes insipidus (NDI) express functional vasopressin renal V2-type receptors" J. Biol. Chem. 265, 15379-15382

30. Jans, D.A., Peters, R., Jans, P. & Fahrenholz, F. (1990) "Ammonium chloride affects receptor number and lateral mobility of the vasopressin V2-type receptor in the plasma membrane of LLC-PK1 renal epithelial cells: role of the cytoskeleton" Exper. Cell Res. 191, 121-128

31. Grünbaum, E-G., Herzog, A., Rascher, W., Fahrenholz, F., Luzius, H., Jans, D.A., Moritz, A. & Hecht, W. (1990) "Genetisch bedingter Diabetes Insipidus Renalis beim Hund" Deutsche Veterinär Medizinische Gesellschaft DVG, Fachgruppe Kleintierkrankheiten 35, 126-152

32. Jans, D.A., Peters, R. & Fahrenholz, F. (1990) "An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin renal-type V2- receptor; an active role for receptor immobilization in down-regulation?" FEBS Lett. 274, 223-226

33. Pearson, D., Nigg, E.A., Nagamine, Y., Jans, D.A. & Hemmings, B.A. (1991) "Mechanisms of cAMP-mediated gene induction; examination of renal epithelial cell mutants affected in the catalytic subunit of the cAMP-dependent protein kinase" Exper. Cell Res. 192, 315-318

34. Jans, D.A., Resink, T.J. & Hemmings, B.A. (1991) "A novel LLC-PK1 renal epithelial cell mutant impaired in in vivo down-regulation of cAMP-mediated hormonal response" Arch. Biochem. Biophys. 285, 377-381

35. Jans, D.A. & Hemmings, B.A. (1991) "cAMP-dependent protein kinase activation affects vasopressin V2-receptor number and internalization in LLC-PK1 renal epithelial cells" FEBS Lett. 281, 267-271

36. Rihs, H-P., Jans, D.A., Fan, H. & Peters, R. (1991) "The rate of nuclear cytoplasmic protein transport is determined by a casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen" EMBO J. 10, 633-639

37. Jans, D.A., Peters, R., Jans, P. & Fahrenholz, F. (1991) "Vasopressin V2-receptor mobile fraction and ligand-dependent adenylate cyclase-activity are directly correlated in LLC- PK1 renal epithelial cells" J. Cell Biol. 114, 53-60

38. Jans, D.A., Ackermann, M., Bischoff, J.R., Beach, D.H. & Peters, R. (1991) "p34cdc2- mediated phosphorylation at T124 inhibits nuclear import of SV40 T-antigen proteins" J. Cell Biol. 115, 1203-1212

39. Luzius, H., Jans, D.A., Jans, P. & Fahrenholz, F. (1991) "Isolation and genetic characterization of a renal epithelial cell mutant defective in vasopressin V2-type receptor binding and function" Exper. Cell Res. 195, 478-484

40. Jans, D.A., Jans, P., Luzius, H. & Fahrenholz, F. (1991) "Monensin resistant LLC-PK1 mutants are affected in recycling of the adenylate cyclase-stimulating vasopressin V2- receptor" Mol. Cell. Endocrinol. 81, 165-174

41. Wartmann, M., Jans, D.A., Parker, P.J., Nagamine, Y., Hemmings, B.A., Jaken, S., Eppenberger, U. & Fabbro, D. (1991) "Overexpression of the alpha-type protein kinase (PK) C in LLC-PK1 cells does not lead to a proportional increase in the induction of two 12- O-tetradecanoyl-phorbol-13-acetate inducible genes" Mol. Biol. of the Cell 2, 491- 502

42. Munn, A.L., Whitfeld, P.R., Bottomley, W., Hudson, G.S., Jans, D.A., Gibson, F. & Cox,G.B. (1991) "The chloroplast ß-subunit allows assembly of the Escherichia coli Fo portion of the energy transducing adenosine triphosphatase" Biochim. Biophys. Acta 1060, 82-88

43. Van den Ouweland, A., Markslag, P., Knoers, N., Ropers, H.H., Poustka, A., Jans, D.A., Fahrenholz, F. & van Oost, B. (1991) "Colocalization of the locus for human nephrogenic diabetes insipidus and the vasopressin type-2 receptor gene in the Xq28 region" Miami Short Reports 1, 20

44. Rosenkranz, A.A., Yachmenev, S.V., Jans, D.A., Serebryakova, N.V., Murav'ev, V.I., Peters, R. & Sobolev, A.S. (1992) "Receptor-mediated endocytosis and nuclear transport of a transfecting DNA construct" Exper. Cell Res. 199, 323-329

45. Luzius, H., Jans, D.A., Grünbaum, E-G., Moritz, A., Rascher, E. & Fahrenholz, F. (1992) "A low affinity vasopressin renal-type V2-receptor in X-linked nephrogenic diabetes insipidus (NDI)" J. Receptor Res. 12, 351-368

46. Jans, D.A., Jans, P., Luzius, H. & Fahrenholz, F. (1992) "N-glycosylation plays a role in biosynthesis and internalization of the adenylate cyclase-stimulating vasopressin V2- receptor of LLC-PK1 renal epithelial cells: an effect of concanavalin A on V2-expression and binding" Arch. Biochem. Biophys. 294, 64-69

47. Jurzak, M., Jans, D.A., Haase, W., Peters, R. & Fahrenholz, F. (1992) "Generation of anti- idiotypic monoclonal antibodies recognizing vasopressin receptors in cultured cells and kidney sections" Exper. Cell Res. 203, 182-191

48. Jans, D. A. (1992) "The mobile receptor hypothesis revisited: a mechanistic role for hormone receptor lateral mobility in signal transduction" Biochim. Biophys. Acta 1113, 271- 276

49. Akhlynina, T.V., Yachmenev, S.V., Jans, D.A., Gulak, P.V., Rosenkranz, A.A., Serebryakova, N.V. & Sobolev, A.S. (1993) "The use of internalizable derivatives of chlorin e6 for increasing its photosensitizing activity" Photochem. Photobiol. 58, 45-48

50. Jans, D.A., Pavo, I. & Fahrenholz, F. (1993) "Oxytocin-induced cAMP-dependent protein kinase activation and urokinase-type plasminogen activator production in LLC-PK1 renal epithelial cells is mediated by the vasopressin V2-receptor" FEBS Lett. 315, 134-138

51. Pavo, I., Jans, D.A., Peters, R., Penke, B. & Fahrenholz, F. (1994) "A vasopressin antagonist that binds to the V2-receptor of LLC-PK1 renal epithelial cells is highly laterally mobile but does not effect ligand-induced receptor immobilization" Biochim. Biophys. Acta 1223, 240-246

52. Jans, D.A. & Jans, P. (1994) "Negative charge at Ser112 of the casein kinase II site flanking the nuclear localization signal of the SV40 large T-antigen is mechanistically important in enhancing nuclear import" Oncogene 9, 2961-2968

53. Jans, D.A. (1994) "Nuclear signalling pathways for extracellular ligands and their membrane- integral receptors ?" FASEB J. 8, 841-847

54. Akhlynina, T, V., Rosenkranz, A. A., Jans, D. A. & Sobolev, A. S. (1995) "Insulin- mediated intracellular targeting enhances the photodynamic activity of chlorin e6" Cancer Res. 55, 1014-1019

55. Jans, D.A. (1995) "Regulation of protein transport to the nucleus by phosphorylation" Biochem. J. 311, 705-716

56. Jans, D.A., Moll, T., Nasmyth, K. & Jans, P. (1995) "Cyclin-dependent kinase-site regulated signal dependent nuclear localization of the SWI5 yeast transcription factor in mammalian cells", J. Biol. Chem. 270, 17064-17067

57. Jans, D.A. (1995) "Phosphorylation-mediated regulation of signal-dependent nuclear protein transport - the'CcN motif'" Membrane Protein Transport 2, 161-199

58. Jans, D.A. & Pavo, I. (1995) "A mechanistic role for polypeptide hormone receptor lateral mobility in signal transduction" Amino Acids 9, 93-109

59. Walaschewski, U., Jans, D.A., Neugebauer-Goedde, D-C. & Peters, R. (1995) "Semi- intact cells for nucleo-cytoplasmic transport studies" Methods in Mol. Cell. Biol. 5, 87-95

60. Crouch, M., Jans, D.A., Simson, L. & Hendry, I.A. (1995) "Interaction of the GTP-binding protein Gi2 with a protein kinase A-like kinase in mouse fibroblasts" Aus. N.Z. J. Med. 25, 831-836

61. Trapani, J.A., Browne, K.A., Smyth, M.J. & Jans, D.A. (1996) "Localization of granzyme B in the nucleus: a putative role in the mechanism of cytotoxic lymphocyte-mediated apoptosis" J. Biol. Chem. 271, 4127-4133

62. Xiao, C-Y., Hübner, S., Elliot, R.M., Caon, A. & Jans, D.A. (1996) "A consensus PK-A site in place of the CcN motif casein kinase II site of SV40 large T-antigen confers PK-A- mediated regulation of nuclear import" J. Biol. Chem. 271, 6451-6457

63. Jans, D.A., Jans, P., Briggs, L.J., Sutton, V. & Trapani, J.A. (1996) "Nuclear transport of granzyme B (fragmentin-2) - dependence on perforin in vivo and cytosolic factors in vitro." J. Biol. Chem. 271, 30781-30789

64. Ymer, S. & Jans, D.A. (1996) "In vivo chromatin structure of the murine interleukin-5 gene region: a new intact cell system" Biotechniques 20, 834-840

65. Hübner, S., Xiao, C-Y., Reid, J., Briggs, L.J., Corrigan, V., Jans, P. & Jans, D.A. (1996) "Regulation of protein transport to the nucleus" Today's Life Science 8, 30-38

66. Jans, D.A. & Hübner, S. (1996) "Regulation of protein transport to the nucleus - the central role of phosphorylation" Physiol. Rev. 76, 651-685

67. Seydel, U. & Jans, D.A. (1996) "Evidence for an inhibitory feedback loop regulating SV40 large T-antigen fusion protein nuclear transport" Biochem. J. 315, 33-39

68. Jans D. A., Briggs, L.J., Gustin, S.E., Jans, P., Ford, S. & Young, I.G. (1997) "A functional bipartite nuclear localization signal in the cytokine interleukin-5" FEBS Lett. 406, 315-320

69. Hübner, S., Xiao, C-Y. & Jans, D.A. (1997) "The protein kinase CK2 site (Ser111/112) enhances recognition of the SV40 large T-antigen nuclear localization sequence by importin" J. Biol. Chem. 272, 17191-17195

70. Xiao, C-Y., Hübner, S. & Jans, D.A. (1997) "SV40 large tumor-antigen nuclear import is regulated by the double-stranded DNA-dependent protein kinase site (serine 120) flanking to the nuclear localization sequence" J. Biol. Chem. 272, 22191-22198

71. Jans D. A., Briggs, L.J., Gustin, S.E., Jans, P., Ford, S. & Young, I.G. (1997) "The cytokine interleukin-5 (IL-5) effects cotransport of its receptor subunits to the nucleus in vitro" FEBS Lett. 410, 368-372

72. Akhlynina, T.V., Jans, D.A., Statsyuk, N.V., Balashova, I.Y., Toth, G., Pavo, I., Rosenkranz, A.A., Rubin, A.B. & Sobolev, A.S. (1997) "Nuclear targeting of chlorin e6 enhances its photosensitising activity" J. Biol. Chem. 272, 20328-20331

73. Efthymiadis, A., Shao, H., Hübner, S. & Jans, D.A. (1997) "Kinetic characterization of the human retinoblastoma protein bipartite nuclear localization sequence in vivo and in vitro: a comparison with the SV40 large T-antigen NLS" J. Biol. Chem. 272, 22134-22139

74. Piller, S.C., Jans, P., Gage, P.W. & Jans, D.A. (1998) "Extracellular HIV-1 Vpr causes a large inward current and cell death in cultured hippocampal neurons: implications for AIDS pathology" Proc. Natl. Acad. Sci. USA 95, 4595-4600

75. Jans, D.A. & Hassan, G. (1998) "Nuclear targeting by growth factors, cytokines and their receptors: a role in signaling ?" BioEssays 20, 400-411

76. Efthymiadis, A., Briggs, L.J. & Jans, D.A. (1998) "The HIV-1 Tat nuclear localization sequence confers novel nuclear import properties" J. Biol. Chem. 273, 1623-1628

77. Trapani, J.A., Jans, P., Smyth, M.J., Froelich, C.J., E.A. Williams, Sutton, V.R. & Jans, D.A. (1998) "Perforin-dependent nuclear entry of granzyme B precedes apoptosis and is not a consequence of nuclear envelope dysfunction" Cell Death and Differentiation 5, 488-496

78. Briggs, L.J., Stein, D., Goltz, J., Corrigan, V.C., Efthymiadis, A., Hübner, S. & Jans, D.A. (1998) "The PK-A site (Ser312) enhances Dorsal nuclear import through facilitating nuclear localization sequence/importin interaction" J. Biol. Chem. 273, 22745-22752

79. Chan, C.K., Hübner, S., Hu, W. & Jans, D.A. (1998) "Mutual exclusivity of DNA binding and nuclear localization signal recognition by the yeast transcription factor GAL4: implications for non-viral DNA delivery" Gene Therapy 5, 1204-1212

80. Jans, D.A., Briggs, L.J., Jans, P., Froelich, C.J., Parasivam, G., Kumar, S., Sutton, V.R. & Trapani, J.A. (1998) "Nuclear targeting of the serine protease granzyme A (fragmentin-1)" J. Cell Sci. 111, 2645-2654

81. Trapani, J.A., Jans, D.A., Jans, P., Smyth, M.J., Browne, K.A. & Sutton, V.R. (1998) "Efficient nuclear targeting and the nuclear consequences of apoptosis induced by granzyme B and perforin are caspase-dependent, but cell death is caspase-independent" J. Biol. Chem. 273, 27934-27938

82. Efthymiadis, A., Dottorini, T. & Jans, D.A. (1998) "A novel system to quantitate nuclear- cytoplasmic flux: kinetics of signal-dependent nuclear protein export" Arch. Biochem. Biophys. 355, 254-261

83. Jans, D.A., Chan, C.K. & Hübner, S. (1998) "Signals mediating nuclear targeting and their regulation; application in drug delivery" Med. Res. Reviews 18, 189-223

84. Jans, D.A. (1998) "Protein targeting to the nuclear compartment" Australian Biochemist 29, 5-10

85. Xiao, C-Y. & Jans, D.A. (1998) "An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import" FEBS Lett. 436, 313- 317

86. Xiao, C-Y., Jans, P. & Jans, D.A. (1998) "Negative charge at the protein kinase CK2 site enhances recognition of the SV40 large tumor-antigen nuclear localization signal by importin; effect of proline near the CK2 site" FEBS Lett. 440, 297-301

87. Chan, C.K. & Jans, D.A. (1999) "Enhancement of polylysine-mediated transferrinfection by nuclear localization sequences (NLSs): polylysine does not function as an NLS" Human Gene Therapy 10, 1695-1702

88. Jans, D.A., Sutton, V.R., Jans, P., Froelich, C.J. & Trapani, J.A. (1999) "Bcl-2 blocks perforin-induced nuclear translocation of granzymes, concomitant with protection against the nuclear events of apoptosis" J. Biol. Chem. 274, 3953-3961

89. Loveland, K.L., Herszfeld, D., Chu, B., D.M., Briggs, L.J., Shakri, R., de Kretser, & Jans, D.A. (1999) "A novel low molecular weight microtubule-associated protein-2 (MAP-2) isoform containing a functional nuclear localization sequence in testis" J. Biol. Chem. 274, 19261-19268

90. Hübner, S., Smith, H.M.S., Hu, W., Chan, C.K., Rihs, H-P., Paschal, B., Raikhel, N.V. & Jans, D.A. (1999) "Plant importin a binds nuclear localization sequences with high affinity and mediates nuclear import independent of importin b" J. Biol. Chem. 274, 22610-22617

91. Piller, S.C., Ewart, G.D., Jans, D.A., Gage, P.W. & Cox, G.B. (1999) "The amino terminal region of VPR from HIV-1 forms ion channels and kills neurons" J. Virol. 73, 4230-4238

92. Lam, M.H.C., Briggs, L.J., Hu, W., Martin, T.J., Gillespie, M.T. & Jans, D.A. (1999) "Importin b recognizes parathyroid hormone-related protein (PTHrP) with high affinity and mediates its nuclear import in the absence of importin a" J. Biol. Chem. 274, 7391- 7398

93. Akhlynina, T.V., Jans, D.A., Statsyuk, N.V., Balashova, I.Y., Toth, G., Pavo, I., Rosenkranz, A.A., Naroditsky, B.S. & Sobolev, A.S. (1999) "Adenoviruses synergize with nuclear localization signals to enhance the nuclear delivery and photodynamic action of internalizable conjugates containing chlorin e6" Int. J. Cancer 81, 737-740

94. Hu, W. & Jans, D.A. (1999) "Efficiency of importin a/b-mediated NLS recognition and nuclear import: differential role of NTF2" J. Biol. Chem. 274, 15820-15827

95. Blink, E.J., Trapani, J.A. & Jans, D.A. (1999) "Perforin-dependent nuclear targeting of granzymes: central role in the nuclear events of granule-exocytosis-mediated apoptosis ?" Immunol. Cell Biol. 77, 206-215

96. Forwood, J.K., Brooks, A., Briggs, L.J., Xiao, C-Y., Jans, D.A. & Vasudevan, S.G. (1999) "The 37 amino acid interdomain of dengue virus NS5 RNA polymerase contains a functional NLS and inhibitory CK2 site" Biochem. Biophys. Res. Commun. 257, 731-737

97. Browne, K.A., Blink, E., Sutton, V.R., Froehlich, C.R., Jans, D.A. & Trapani, J.A. (1999) "Cytosolic delivery of granzyme B by bacterial toxins: evidence that endosomal disruption, in addition to transmembrane pore formation, is an important function of perforin" Mol. Cell. Biol. 19, 8604-8615

98. Percipalle, G., Butler, P.J.G., Finch, J.T., Jans, D.A. & Rhodes, D. (1999) "Nuclear localisation signal recognition causes release of importin-alpha from aggregates in the cytosol" J. Mol. Biol. 292, 263-273

99. Jans, D.A., Johnson-Saliba, M. & Lam, M.H.C. (1999) "A mounting chamber for confocal microscopy" Microscopy Today 99-9, 28-29

100. Browne, K.A., Johnstone, R.W., Jans, D.A. & Trapani, J.A. (2000) "Filamin (280 kDa actin-binding protein is a caspase substrate, and is also cleaved by the cytotoxic T lymphocyte protease granzyme B during apoptosis" J. Biol. Chem. 275, 39262-39266

101. Chan, C.K. & Jans, D.A. (1999) "Synergy of importin a recognition and DNA binding by the yeast transcriptional activator GAL4" FEBS Lett. 462, 221-224

102. Jans, D.A., Lam, M.H.C. & Xiao, C-Y. (2000) "Nuclear targeting signal recognition: central control point of nuclear protein transport ?" BioEssays 22, 532-544

103. Briggs, L.J., Johnstone, R.W., Elliot, R.M., Xiao, C-Y., Dawson, M., Trapani , J.A. & Jans, D.A. (2000) "Novel properties of the CK2-site regulated NLS of the interferon-induced nuclear factor IFI 16" Biochem. J. 353, 69-77

104. Johnson-Saliba, M., Siddon, N.A., Clarkson, M.J., Tremethick, D.J. & Jans, D.A. (2000) "Distinct importin recognition properties of histones and chromatin assembly factors" FEBS Lett. 467, 169-174

105. Schedlich, L.A., Le Page, S.L., Firth, S.M., Briggs, L.J., Jans D. A. & Baxter, R.C. (2000) "Nuclear import of insulin-like growth factor binding protein-3 (IGFBP-3) and IGFBP-5 is mediated by the importin b subunit" J. Biol. Chem. 275, 23462-23470

106. Sobolev, A.S., Jans, D.A. & Rosenkranz, A.A. (2000) "Targeted intracellular delivery of photosensitizers" Prog. Biochem. Mol. Biol. 73, 51-90

109. Jans, D.A., P. Jans, T. Juelich, L. J. Briggs, L.J., Xiao, C-Y. & Piller, S. C. (2000) "Intranuclear binding by the HIV-1 regulatory protein Vpr is dependent on cytosolic factors" Biochem. Biophys. Res. Commun. 270, 1055-1062

110. Jans, D.A. (2000) "Signals targeting proteins to the nucleus and their application for drug delivery" Recent Advances and Research Updates in Medicine 1, 45-67

111. Lam, M.H.C. & Jans, D.A. (2000) "Buying confocal microscopes - a fly-eye's view" Microscopy Today 2000-3, 8

112. Lam, M.H.C., Thomas, R.J., Martin, T.J., Gillespie, M.T. & Jans, D.A. (2000) "Nuclear and nucleolar localization parathyroid hormone-related protein (PTHrP)" Immunol. Cell Biol. 78, 395-402

113. Chan, C.K., Senden, T. & Jans, D.A. (2000) "Supramolecular structure and nuclear targeting efficiency determine the enhancement of transfection by NLS-containing polylysine" Gene Therapy 7, 1690-1697

114. Rosenkranz, A.A., Jans, D.A. & Sobolev, A.S. (2000) "Targeted delivery of photosensitizers to enhance photodynamic efficiency" Immunol. Cell Biol. 78, 452-464

115. Lam, M.H.C., Henderson, B., Gillespie, M.T. & Jans, D.A. (2001) "Dynamics of leptomycin B-sensitive nucleocytoplasmic flux of parathyroid hormone-related protein" Traffic 2, 812-829

116. Chan, C.K. & Jans, D.A. (2001) "Enhancement of MSH-receptor- and GAL4-mediated gene transfer by switching the nuclear import pathway" Gene Therapy 8, 166-171

117. Bird, C.H., Blink, E.J., Hirst, C.E., Buzza, M.S., Steele, P.M., Sun, J., Jans, D.A. & Bird, P.I. (2001) "Nucleocytoplasmic Distribution of the Ovalbumin Serpin PI-9 Requires a Non-Conventional Nuclear Import Pathway and the Export Factor Crm1" Mol. Cell. Biol. 21, 5396-5407.

118. Forwood, J.K., Lam, M.H.C. & Jans, D.A. (2001) "Nuclear import of the CREB and AP-1 transcription factors is dependent on importin b1 and Ran, and independent of importin a" Biochemistry 40, 5208-5217

119. Johnson-Saliba, M. & Jans, D.A. (2001) " Gene therapy: optimising DNA delivery to the nucleus" Curr. Drug Targets 2, 371-399

120. Wangerek, L.A., Dahl, H-H.M., Senden, T.J., Carlin, J.B., Jans, D.A., Ioannou, P.A., Williamson, R. & Forrest, S.M. (2001) "Atomic force microscopy imaging of DNA-cationic liposome complexes optimised for gene transfection into neuronal cells" J. Gene Medicine 3, 72-81

121. Hübner, S., Jans, D.A. & Drenckhahn, D. (2001) "Roles of cytoskeletal and junctional plaque proteins in nuclear signaling" Int. Rev. Cytol. 208, 207-265.

122. Johansson, M., Brooks, A., Jans, D.A. & Vasudevan, S.G. (2001) "A small region of Dengue Virus encoded NS3 confers interaction with both the nuclear transport receptor importin-b and the viral RNA Dependent RNA Polymerase NS5" J. Gen. Virol. 82, 735-745.

123. Lam, M.H.C., Hu, W., Xiao, C-Y., Gillespie, M.T. & Jans, D.A. (2001) "Molecular dissection of the importin b1-recognized nuclear targeting signal of parathyroid hormone-related protein (PTHrP)" Biochem. Biophys. Res. Commun. 282, 629-634.

124. Forwood, J.K., Harley, V. & Jans, D.A. (2001) "The C-terminal nuclear localization signal of the SRY HMG domain mediates nuclear import through importin b1" J. Biol. Chem. 276, 46575-46582.

125. Lixin, R., Efthymiadis, A., Henderson, B. & Jans, D.A. (2001) "Novel properties of the nucleolar targeting signal of human angiogenin" Biochem. Biophys. Res. Commun. 284, 185-193

126. Preiss, S., Argentaro, A., Clayton, A., John, A., Jans, D.A., Ogata, T., Nagai, T., Barroso, I., Schafer, A. & Harley, V. (2001) "Compound effects of point mutations causing campomelic dysplasia/autosomal sex reversal upon SOX9 structure, nuclear transport , DNA binding and transcriptional activation" J. Biol. Chem. 276, 27864-27872.

127. Vasudevan, S.G., Johansson, M., Brooks, A.J., Llewellyn, L.E. & Jans, D.A. (2001) "Characterisation of inter- and intra-molecular interactions of the dengue virus RNA dependent RNA polymerase as potential drug targets." Il Farmaco 56, 33-36.

128. Catimel, B., Teh, T., Fontes, M.R.M., Jennings, I.G., Jans, D.A., Howlett, G. J., Nice, E.C. & Kobe, B. (2001) "Biophysical characterization of interactions involving importin-a during nuclear import" J. Biol. Chem. 276, 34189-34198.

129. Brooks, A.J., Johansson, M., Criswell, E., Jans, D.A., & Vasudevan, S.G. (2002) "The interdomain region of Dengue NS5 protein interacts with NS3 and host proteins" Dengue Bulletin 26, in the press

130. Hübner, S., Jans, D.A., Xiao, C-Y., John, A.P. & Drenckhahn, D. (2002) "Signal- and importin-dependent nuclear targeting of the kidney anion exchanger 1-binding protein kanadaptin" Biochem. J. 361, 287-296

131. Hariton-Gazal, E., Feder, R., Mor, A., Graessmann, A., Brack-Werner, R., Jans, D.A., Gilon, C. & Loyter, A. (2002) "Targeting of non karyophilic cell-permeable peptides into the nuclei of intact cells by covalently attached nuclear localization signals" Biochemistry 41, 9208-9214.

132. Lam, M.H.C., Thomas, R.J., Loveland, K.L., Schilders, S., Min Gu, Martin, T.J., Gillespie, M.T. & Jans, D.A. (2002) "Transport of parathyroid hormone related protein (PTHrP) to the nucleus is dependent on microtubules" Mol. Endocrinol. 16, 390-401

133. Chan, C.K. & Jans, D.A. (2002) "Using nuclear targeting signals to enhance non-viral gene transfer" Immunol. Cell Biol. 80, 119-130

134. Forwood, J.K. & Jans, D.A. (2002) "Nuclear import of the telomere elongation suppressor TRF1; inhibition by importin a" Biochemistry 41, 9333-9348

135. Henderson, M.J., Russell, A.J., Hird, S., Munoz, M., Clancy, J.L., Lehrbach, G.M., Calanni, S.T., Jans, D.A., Sutherland, R.L. & Watts, K.W. (2002) "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response" J. Biol. Chem. 277, 26468-26478.

136. Brooks, A.J., Johansson, M., John, A.J., Xu, Y., Jans, D.A., & Vasudevan, S.G. (2002) "The interdomain region of Dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin b1 and importin a?b-recognised nuclear localisation signals (NLSs)" J. Biol. Chem. 277, 36399-36407.

137. Piller, S., Caly, L. & Jans, D.A. (2002) "Nuclear import of the pre-integration complex (PIC): the Achilles heel of HIV ?" Curr. Drug Targets, in the press

138. Fineberg, K., Fineberg, T., Graessmann, A., Luedtke, N.W., Tor, Y., Rui, L., Jans, D.A. & Loyter, A. (2003) "Inhibition of Rev-arginine rich motif-mediated nuclear import by the HIV-1 rev response element" Biochemistry, in the press

139. Trapani, J.A., Sutton, V.R., Thia, K.Y.T., Qin Li, U., Froelich, C.J., Jans, D.A., Sandrin, M.S., & Browne, K.A. (2003) "A clathrin/dynamin- and mannose-6-phosphate receptor-independent pathway for granzyme B-induced cell death" J. Cell Biol., in the press