Professor David A Jans

BSc (Hons, University of Melbourne), PhD (JCSMR, Canberra)

Telephone:   +61 3 9902 9341 Facsimile:    +61 3 9902 9500 Location:      Room 146, Building 77, Clayton Email:        David.Jans@med.monash.edu.au

  General Profile:

David Andrew Jans is originally from Melbourne. After graduating (BSc. Hons) from the University of Melbourne Microbiology Dept. in 1980, he joined the Dept. of Biochemistry at the John Curtin School of Medical Research (JCSMR) to carry out his Ph.D. studies with Graeme Cox and Frank Gibson on bacterial ATPase (completed 1984). He then took up a research scientist position at the Friedrich Miescher Institut in Basel (Switzerland), followed by a visiting fellowship at the Max Planck Institut für Biophysik in Frankfurt am Main (Germany), working in the area of phosphorylation and signal transduction in mammalian cells. In 1990, he became a Senior Scientist at the Institut für Medizinische Physik und Biophysik, Westfälische Wilhelms-Universität in Münster (Germany), turning his focus to the use of microscopic techniques to investigate transport processes. He returned in 1993 to the JCSMR (Division of Biochem. & Mol. Biol.), initially as a Fellow, to establish the Nuclear Signalling Laboratory, with a strong focus on the processes of protein transport into the nucleus. He rose to Senior Fellow in 1998, Full Professor in 2000, and since 1998 also holds a conjoint teaching appointment (Associate Professor/Professor) at the James Cook University of North Queensland (Townsville, Australia). He has been at Monash since 2002, as an NHMRC Senior Principal Research Fellow (SPRF), with a personal chair.

Prof. Jans has made a number of contributions to the general area of biological chemistry/molecular biology, and in particular how phosphorylation regulates the trafficking of viral, cancer regulatory and other cellular proteins to the nucleus in eukaryotic cells, have had a major impact over a number of years in the field, as evidenced by the many, many citations of his work (c. 2800 citations - he is listed as no. 1405 of the most cited scientists in the general area of biochemistry and biology, including 11 publications that have been cited over 100 times, with a H-index of 46). He has close to 200 primary research publications, and 40 peer-reviewed review articles), including publications in eminent journals such as J. Biol. Chem. (total of 30 papers), Proc. Natl. Acad. Sci. USA (3), EMBO J. (3), J. Cell Biol. (3), Biochem. J. (13), FASEB J. (4), Oncogene (3), Cancer Research (2), Retrovirology (2), J. Virol. (8), Traffic (7), Mol. Cell. Biol. (2), Mol. Biol. of the Cell (3) and Nature Reviews Genetics. His international profile is further emphasized by the number of invitations to speak at international meetings (eg. ASCB, EMBO workshops) including 7 plenary talks (eg. 9th International Conference "Drug and Gene-based Therapeutics", Crete; Proteins Killing Tumor Cells Leiden, Holland), and awards (eg. IRPC (International Research Progress Council) Eminent Scientist of the Year, 1999, and Danny Thomas Lecture Series Visiting Professor, St. Jude Children's Research Hospital, Memphis, 2005), and the invitations to serve as journal editor (Biochem. J., Open AIDS J.), and Committee member of the International Photodynamic Association.

The main focus of Prof. Jans' work has been to delineate the mechanisms regulating protein transport to the nucleus, which is central to many important cellular processes such as differentiation, transformation, signal transduction and the regulation of transcription. His signature approach has been to use biophysical approaches (eg. quantitative confocal laser scanning microscopy - CLSM/image analysis) together with microinjection or reconstituted in vitro systems to examine nuclear protein import kinetics at the single cell level, as well as various binding approaches utilizing fluorescent measurements (eg. fluorescence polarization/ fluorography/ALPHAscreen) to analyse the initial step of nuclear transport - that of targeting signal (nuclear localization sequence - NLS) recognition by members of the cellular importin (IMP) nuclear import receptor/transport. He also pioneered the use of the technique of fluorescence recovery after photobleaching (FRAP) to determine nuclear transport kinetics in living cells.

Using these predominantly quantitative approaches, the Jans lab. has made a number of major contributions to knowledge of nuclear transport, especially with respect to its regulation (see below). For example, his lab. showed that whilst nuclear localisation is NLS-dependent, phosphorylation at sites near NLSs can either enhance or inhibit NLS-dependent nuclear transport; hormonal signals can modulate gene expression through regulating phosphorylation at such sites, thereby specifically controlling the nuclear entry of particular TFs. His lab. defined for the first time the mechanism by which NLS-dependent nuclear protein transport can be enhanced by phosphorylation; through phosphorylation enhancing IMP-NLS interaction in the case of the Drosophila morphogen Dorsal and simian virus SV40 large tumour-antigen (T-ag). His lab. has worked intensively on the nuclear import pathways used by viral proteins, and in particular those of the HIV-1 and Dengue viruses, the causative agents of AIDS and Dengue fever, respectively, as well as Rabies virus, and viruses causing respiratory disease/asthma exacerbation such as respiratory syncytial virus and rhinovirus. The strategy has been to define the import pathways of these proteins and the regulation thereof, and thereby identify potential targets for the future development of anti-virals to block viral infection. The lab. has also used knowledge of nuclear protein import in order to target molecules of interest to the nucleus in a research or clinical setting. This has included the nuclear delivery of DNA of relevance to gene therapy applications (eg. the introduction of normal gene copies into appropriate cells harbouring a genetically conferred error of metabolism), or alternatively, of toxins to the nucleus of tumour cells with relevance to anti-cancer therapy applications. The approach here has been to use modular conjugate molecules containing modified NLSs with these applications in mind, the more recent interest work being a tumour cell specific nuclear targeting signal from chicken anaemia virus (CAV) VP3 protein, which intriguingly shows phosphorylation-regulated nuclear localization in tumour cells, but not normal cells.

Research Links:

Nuclear Signalling

Review Articles in Peer-Reviewed Journals (2003-)

R18. Fulcher, A.J. and Jans, D.A. (2003) “The HIV-1 Tat transactivator protein: a therapeutic target ? IUBMB Life 55, 669-680               

 

R19. Piller, S., Caly, L. & Jans, D.A. (2003) “Nuclear import of the pre-integration complex (PIC): the Achilles heel of HIV ?” Curr. Drug Targets 4, 409-429

                       

R20. Jans, D.A., Thomas, R.J. & Gillespie, M.T. (2003) “Parathyroid hormone-related protein (PTHrP): a nucleocytoplasmic shuttling protein with distinct paracrine, and intracrine roles” (Editor G. Litwack), Academic Press, Vitamins and Hormones 66, 345-384  

                       

R21. Johnson, H., Subramaniam, P., S., Olnes, S. & Jans, D.A. (2004) "Trafficking and signaling pathways of nuclear localizing protein ligands and their receptors BioEssays 26, 993-1004      

           

R22. Oro, C. and Jans, D.A. (2004) “The tumour specific pro-apoptotic factor apoptin (VP3) from Chicken Anaemia Virus” Curr. Drug Targets 5, 159-170

                                               

R23. Hogarth, C., Itman, C., Jans, D.A. & Loveland, K.L. (2005) “Regulated nucleocytoplasmic transport in spermatogenesis: a driver of cellular differentiation ? BioEssays 27, 1011-1025     

                                               

R24. Poon, I.K.H., & Jans, D.A. (2005) “Regulation of nuclear transport: central role in development and transformation ?” Traffic 6, 173-186            ⁺Citations 81

                                               

R25. Glover, D., Lipps, H-J. & Jans, D.A. (2005) “Non-viral approaches towards safe, stable therapeutic gene expression in humans” Nature Reviews Genetics 6, 299-310              

                                               

R26. Martin, F., Roth, D. M., Jans, D. A., Pouton, C. W., Partridge, L.J., Monk, P.N. & Moseley. G. W. (2005) “Tetraspanins in viral infections: a fundamental role in viral biology ?” J. Virol. 79, 10839-10851    

                                               

R27. Alvisi, G., Poon, I.K.H. & Jans, D.A. (2006) “Tumor specific nuclear targeting: promises for anti-cancer therapy” Drug. Res. Updates 6, 40-50      I.F. = 4.4

                                               

R28. Ghildyal, R., Ho, A. & Jans, D.A. (2006) “Central role of the Respiratory Syncytial Virus Matrix protein in infection” FEMS Microbiol. Rev. 30, 692-705 

                                               

R29. Rawlinson, C.M., Pryor, M.J., Wright, P.J., & Jans, D.A. (2006) “Dengue virus RNA polymerase NS5 as a therapeutic target” Curr. Drug Targets 7, 1623-1638

                                               

R30. Wagstaff, K.M., & Jans, D.A. (2006) “Protein transduction: cell penetrating peptides and their therapeutic applications” Curr. Medicinal Chem. 13, 1371-1387 

                       

R31. Hearps, A., & Jans, D.A. (2007) “Regulating the functions of the HIV-1 matrix protein” AIDS Research and Human Retroviruses 23, 341-346      I.F. = 2.3

             

R32. Holt, J.E., Ly, J.D., Efthymiadis, A., Loveland, K.L., Hime, G. & Jans, D.A. (2007) “Regulation of nuclear import during differentiation; the IMP a gene family and spermatogenesis” Curr. Genomics 8, 323-334.

             

R33. Pouton, C.W. Wagstaff, K.M., Roth, D.M. & Jans, D.A. (2007) “Targeted delivery to the nucleus” (Lim, C., ed.) in  Advanced Drug Delivery Reviews (Protein Targeting Protocols) 59, 698-717 [Featured on Journal Cover]

             

R34. Wagstaff, K.M. & Jans, D.A. (2007) “Nucleocytoplasmic transport of DNA: enhancing non-viral gene transfer” Biochem. J. 406, 185-202

             

R35. Alvisi, G., Ghildyal, R., Rawlinson, S. & Jans, D.A. (2007) “Regulated nucleocytoplasmic trafficking of viral gene products: a therapeutic target ?” Biochim. Biophys. Acta Proteins and Proteomics 1784, 213-227

             

R36. Glover, D.J., Glouchkova, L., Lipps, H.J. & Jans, D.A. (2007) “Overcoming cellular barriers to safe, sustained and efficient non-viral gene therapy” Advances in Gene, Molecular and Cell Therapy 1, 126-140.

             

R37. Jans, D.A. & Wagstaff, K.M. (2007) “Getting to the heart of a tumour cell” PHARMA Focus Asia 5, 21-24

             

R38. Wagstaff, K.M. & Jans, D.A. (2009) “Importins and Beyond: Non-Conventional Nuclear Transport Mechanisms” Traffic 10, 1188-1198

             

R39. Wagstaff, K.M. & Jans, D.A. (2009) “Nuclear Drug Delivery to Target Tumour Cells” Eur. J. Pharmacol., in press (Ms. No. EJP-30887R1)

 

R40. Itman, C., Miyamoto, Y., Young, J, Jans, D. A. & Loveland, K. L. (2009) “Nucleocytoplasmic Transport as a Driver of Mammalian Gametogenesis” Seminars in Cell and Developmental Biology 20, 607–619

Full Publications in Refereed Journals (2003-)

                       

120. Harley, V.R., Layfield, S., Mitchell, C., Forwood, J.K., John, A.J., Briggs, L.J., McDowall, S. & Jans, D.A. (2003) “Defective importin-b recognition and nuclear import of the sex-determining factor SRY are associated with XY sex reversing mutations” Proc. Natl. Acad. Sci. USA 100, 7045-7050  [Featured in Science News of the Week: Science 302, 2050, 2003: “In sex reversal, protein deterred by nuclear barrier”]    

 

121. Fontes, M.R.M, The, T., Jans, D.A., Brinkworth, R.I. & Kobe, B. (2003) “Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-a“ J. Biol. Chem. 278, 27981-27987  

             

122. Fineberg, K., Fineberg, T., Graessmann, A., Luedtke, N.W., Tor, Y., Lixin, R., Jans, D.A. & Loyter, A. (2003) “Inhibition of Rev-arginine rich motif-mediated nuclear import by the HIV-1 rev response element“ Biochemistry USA 42, 2625-2633

                       

123. Trapani, J.A., Sutton, V.R., Thia, K.Y.T., Qin Li, U., Froelich, C.J., Jans, D.A., Sandrin, M.S., & Browne, K.A. (2003) “A clathrin/dynamin- and mannose-6-phosphate receptor-independent pathway for granzyme B-induced cell death” J. Cell Biol. 160, 223-233    [Featured in J. Cell Biol. Comment: J. Cell Biol. 160,155-156, 2003: “Endocytosing the death sentence”]              

                       

124. Fontes, M.R.M, The, T., Toth, G., John, A., Pavo, I., Jans, D.A. & Kobe, B. (2003) “The role of flanking sequences and phosphorylation in the recognition of SV40 T-antigen nuclear localization sequence by importin-a“ Biochem. J. 375, 339-349  

                       

125. Rosenkranz, A.A., Lunin, V.G., Gulak, P.V., Sergienko, O.V., Shumiantseva, M.A., Voronina, O.I., Gilyazova, D.G., John, A.P., Kofner, A.A., Mironov, A.F., Jans, D.A. & Sobolev, A.S. (2003) “Recombinant modular transporters for cell-specific nuclear delivery of locally-acting drugs enhance photosensitizer activity” FASEB J. 17, 1121-1124

                       

126. Keough, R.A., Macmillan, E.M., Lutwyche, J.K., Gardner, J.M., Tavner, F.J., Jans, D.A., Henderson, B.R., & Gonda, T.J. (2003) “Myb-binding protein 1a is a nucleocytoplasmic shuttling protein that utilises CRM1-dependent and -independent nuclear export pathways” Exper. Cell Res. 289, 108-123    

             

127. Schedlich, L.J., Nilsen, T., John, A.P., Jans, D.A. & Baxter, R.C. (2003) “Phosphorylation of Insulin-like Growth Factor Binding Protein‑3 (IGFBP‑3) by DNA-dependent Protein Kinase reduces ligand binding and enhances nuclear accumulation” Endocrinol. 144, 1984-

             

128. Argentaro, T., Sim, H., Kelly, S., Preiss, S., Clayton, A., Jans, D.A. & Harley, V.R. (2003) “A SOX9 defect of calmodulin-dependent nuclear import in campomelic dysplasia/ autosomal sex reversal” J. Biol. Chem. 278, 33839-33847  [Featured on Journal Cover: J. Biol. Chem. 278(36)]

             

129. Baliga, B.C., Colussi, P.A., Read, S.H., Dias, M.M., Jans, D.A. & Kumar, S. (2003) “Role of prodomain in importin-mediated nuclear localization and activation of caspase-2” J. Biol. Chem. 278, 4899-4905        

                       

130. Rosenkranz, A.A., Lunin, V.G., Sergienko, O.V., Gilyazova, D.G., Voronina, O.I., Jans, D.A., Kofner, A.A., Shumiantseva, M.A., Mironov, A.F. & Sobolev, A.S. (2003) “Targeted intracellular site-specific drug delivery: photosensitizer targeting to melanoma cell nuclei” Russ. J. Genet. 39, 198-206    I.F. = 0.1

                       

131. Catanzariti, A-M., Soboleva, T.A., Jans, D.A., Board, P.G. & Baker, R.T. (2004) “An efficient system for high-level expression and easy purification of authentic recombinant proteins” Prot. Sci. 13, 1331-1339               

                       

132. Rosenkranz, A.A., Nabatnikov, P.A., Aliev, R.A., Jans, D.A., & Sobolev, A.S. (2004) “Enhancement of cytotoxic action by targeted delivery of alpha-emitter, 211-astatine, into the nuclei of human hepatoma cells” Molec. Med. 2, 55-63

                       

133. Poon I.K.H., Oro, C., Dias, M.M., Jingpu, Z., & Jans, D.A. (2005) “A tumour-cell specific nuclear targeting signal within Chicken Anaemia Virus Vp3/Apoptin” J. Virol. 79, 1339-

                       

134. Soboleva, T.A., Jans, D.A., Johnson-Saliba, M., & Baker, R.T. (2005) “Nuclear-cytoplasmic shuttling of the oncogenic mouse UNP/USP4 deubiquitylating enzyme” J. Biol. Chem. 280, 745-752        

                       

135. Blink, E.J., Jiansheng, Z., Hu, W., Calanni, S.T., Trapani, J.A., Bird, P.I., & Jans, D.A. (2005) Interaction of the nuclear localising cytolytic granule serine protease granzyme B with importin a or b: modulation by the serpin inhibitor PI-9” J. Cell. Biochem. 95, 598-610

                       

136. Hu, W., Kemp, B., & Jans, D.A. (2005) “Kinetic Properties of Nuclear Transport Conferred by the Retinoblastoma (Rb) NLS” J. Cell. Biochem. 95, 782-793        

                       

137. Sunn, K.L., Eisman, J.A., Gardiner, E.M. & Jans, D.A. (2005) “FRAP analysis of nucleocytoplasmic dynamics of the vitamin D receptor splice variant VDRB1: preferential targeting to nuclear speckles” Biochem. J. 388, 509-514

                       

138. Caly, L., Wang, B., Mikhail, M., Gill, M. J., Beckholdt, B., Salemi, M., Jans, D.A., Piller, S.C. & Saksena, N.K. (2005) “Evidence for host driven selection of the HIV-1 vpr gene in vivo during HIV disease progression in a transfusion acquired cohort” AIDS Research and Human Retroviruses 21, 728-733                   

                       

139. Wagstaff, K.M., Dias, M.M., Alvisi, G. & Jans, D.A. (2005) “Quantitative analysis of protein-protein interactions by native page/fluorimaging” J. Fluorescence 15, 469-474   

                       

140. Hogarth, C., Calanni, S.T., Jans, D.A. &  Loveland, K.L. (2005) “Importin a mRNAs have distinct expression profiles during spermatogenesis” Developmental Dynamics 235, 253-262         

                       

141. Young, D.M., Jonnalagada, J., Gatei, M., Jans, D.A., Meyn, S. & Khanna, K.K. (2005) “Identification of the domains of ATM required for nuclear localisation and chromatin association” J. Biol. Chem. 280, 27587-27594   

                       

142. Poon I.K.H., Oro, C., Dias, M.M., Jingpu, Z., & Jans, D.A. (2005) “Apoptin nuclear accumulation is modulated by a Crm1-recognised nuclear export signal that is active in normal but not tumor cells” Cancer Res. 65, 7059-7064 (PRIORITY REPORT) 

                       

143. Alvisi, G., Jans, D.A., Guo, J., Pinna, L.A., & Ripalti, A. (2005) “A protein kinase CK2 site flanking the nuclear targeting signal enhances nuclear transport of human CMV ppUL44” Traffic 6, 1002-1013          

                       

144. Ghildyal, R., Ho, A., Wagstaff, K. M., Dias, M.M., Barton, C.L., Jans, P., Bardin, P, & Jans, D.A. (2005) “Nuclear import of the respiratory syncytial virus matrix protein is mediated by importin b1 independent of importin a" Biochemistry USA 44, 12887-12895     

                       

145. Loveland, K.L., Hogarth, C., Szczepny, A., Prabhu, S.M. & Jans, D.A. (2006) “Expression of Nuclear Transport Importins beta 1 and beta 3 Is Regulated During Rodent Spermatogenesis” Biol. of Reprod. 74, 67-74                

                       

146. Wagstaff, K. M. & Jans, D.A. (2006) “Intramolecular masking of nuclear localization signals; analysis of importin binding using a novel alphascreen-based method” Anal. Biochem. 348, 49-56

                       

147. Huebner, S., Eam, J.E., Huebner, A. & Jans, D.A. (2006) “Laminopathy-inducing lamin A mutants can induce redistribution of lamin binding proteins into nuclear aggregates” Exper. Cell Res. 312, 171-183

                       

148. Huebner, S., Eam, J.E., Wagstaff, K.M. & Jans, D.A. (2006) “Quantitative analysis of localisation and nuclear aggregate formation induced by GFP-lamin A mutant proteins in living HeLa cells” J. Cell Biochem. 98, 810-826

                       

149. Forwood, J.K. & Jans, D.A. (2006) “Quantitative analysis of DNA-protein interactions using double-labelled native gel electrophoresis and fluorimaging” Electrophoresis 27, 3166-3170

                       

150. Hearps, A.C. & Jans, D.A. (2006) “HIV-1 Integrase is capable of targeting DNA to the nucleus via an importin a/b dependent mechanism” Biochem. J. 398, 475-484

                       

151. Janssen, K., Hoffman, T.G, Jans, D.A., Hay, R.T., Schulze-Osthoff, K. & Fischer, U. (2006) “Apoptin is modified by SUMO conjugation and targeted to promyelocytic leukemia protein nuclear bodies” Oncogene 26, 1557-1566                       

                       

152. Alvisi, G., Ripalti, A., Ngankeu, A., Giannandrea, M., Caraffi, S.G., Dias, M.M. & Jans, D.A.  (2006) “HMCV DNA polymerase catalytic subunit pUL54 possesses independently acting nuclear localization and ppUL44 binding motifs” Traffic 7, 1322-1332      

                       

153. Goffin, L., Vodala, S., Fraser, C., Ryan, J., Timms, M., Meusburger, S., Catimel, B., Nice, E.C., Silver, P.A., Xiao, C-Y., Jans D.A. & Gething, M-J.H. (2006) “The unfolded protein response transducer Ire1p contains a nuclear localisation sequence recognised by multiple b importins” Mol. Biol. of the Cell 17, 5309-5323

                       

154. Alvisi, G., Jans, D.A. & Ripalti, A. (2006) "HCMV DNA polymerase processivity factor ppUL44 dimerizes in the cytosol before translocation to the nucleus" Biochemistry USA 45, 6866-6872

                       

155. Wagstaff, K. M., Glover, D.J., Tremethick, D.J. & Jans, D.A. (2007) “Histone mediated transduction as an efficient means for gene delivery” Molecular Therapy 15, 72it1-731

                       

156. Elliot, D.A., Kim, W.S., Jans, D.A. & Garner, B. (2007) “Apoptosis induces neuronal apolipoprotein-E synthesis and localization in apoptotic bodies” Neuroscience Lett. 416, 206-210

                       

157. Roth, D.M., Moseley, G.W., Glover, D., Pouton, C.W. & Jans, D.A. (2007) “A microtubule-facilitated nuclear import pathway for cancer regulatory proteins” Traffic 8, 673–686

                       

158. Hogarth, C., Jans, D.A. &  Loveland, K.L. (2007) “Subcellular distribution of importins correlates with germ cell maturation” Developmental Dynamics 236, 2311-2320

                       

159. Pryor, M.J., Butcher, R.E., Rawlinson, S.M., Barton, C.L., Wen, D., Vasudevan, S., Wright, P.J., Jans, D.A.* & Davidson, A.D. (2007) “Nuclear localization of Dengue NS5 through its importin a/b–recognized NLS is integral to the virus life cycle” Traffic 8, 795-807  *Corresponding author

                       

160. Moseley, G.W., De Jesus, M.A., Roth, D.M., Leyton, D.L., Filmer, R.P., Pouton, C.W. & Jans, D.A. (2007) “Dynein light chain association sequences can facilitate nuclear protein import” Mol. Biol. Of the Cell 18, 3204-3213

                       

161. Alvisi, G., Musiani, D., Jans, D.A. & Ripalti, A. (2007) “An Importin a/b–recognized Bipartite Nuclear Localization Signal Mediates Targeting of the Human Herpes Simplex Virus Type 1 DNA Polymerase Catalytic Subunit pUL30” Biochemistry USA 46, 9155-9163

                       

162. Conway-Campbell, B.L., Wooh, J., Brooks, A.J., Gordon, D., Brown, R., Lichanska, A.M., Chin, H-S., Barton, C.L., Boyle, G.M., Parsons, P.G., Jans, D.A. & Waters, M. (2007) “Nuclear targeting of the growth hormone receptor results in dysregulation of cell proliferation and tumorigenesis” Proc. Natl. Acad. Sci. USA 104, 13331-13336

                       

163. Hearps, A.C., Pryor, M.J., Kuusisto, H.V., Rawlinson, S.M., Piller, S.C. & Jans, D.A. (2007) “Limited ability of the biarsenical dye LUMIO™ to specifically detect tetracysteine-containing proteins within living cells” J. Fluor. 17, 593-597 [Featured on Journal Cover]

                       

164. Moseley, G.W., Filmer, R.P., DeJesus, M.A. & Jans, D.A. (2007) “Nucleocytoplasmic distribution of Rabies Virus P-protein is regulated by phosphorylation adjacent to C-terminal nuclear import and export signals” Biochemistry USA 46, 12053-12061

                       

165. Hearps, A.C., Wagstaff, K.., Piller, S.C. & Jans, D.A. (2007) “The N-terminal basic domain of the HIV-1 matrix protein lacks a conventional nuclear localisation sequence but is required for DNA binding and protein self-association” Biochemistry USA 47, 2199-2210

 

166. Kim, W.S., Elliott, D.A., Kockx, M., Kritharides, L., Rye, K-A., Jans, D.A. & Garner, B. (2007) “Analysis of apolipoprotein-E nuclear localization using green fluorescent protein and biotinylation approaches” Biochem. J. 409, 701-709

                       

167. Ruddock-D’Cruz, N.T., Prashadkumar, S., Wilson, K.J., Heffernan, C., Cooney, M.A., French, A.J., Jans, D.A., Verma, P.J., & Holland, M.K. (2008) “Dynamic changes in localization of chromobox (CBX) family members during the maternal to embryonic transition” Mol. Reprod. Dev. 75, 477-488

                       

168. Elliott, D.A., Kim, W.S., Jans, D.A.  & Garner, B. (2008) “Macrophage apolipoprotein-E knockdown modulates caspase-3 activation without altering sensitivity to apoptosis” Biochim. Biophys. Acta General Subjects 1780, 145-153

                       

169. Maddika, S., Wiechec. E., Ande, S.R., Poon, I.K., Fischer, U., Wesselborg, S., Jans, D.A., Schulze-Osthoff, K. & Los, M. (2008) “Interaction with PI3 kinase contributes to the cytotoxic activity of Apoptin” Oncogene 27, 3060-3065

                       

170. Wagstaff, K. M., Fan, J.Y., de Jesus, M.A., Tremethick, D.J. & Jans, D.A. (2008) “Efficient gene delivery using reconstituted chromatin enhanced for nuclear targeting” FASEB J. 22, 2232-2242

                       

171. Dowling, D., Nasr-Esfahani, S., Tan, C.H., O'Brien, K., Howard, J.L., Jans, D.A., Purcell, D.F.J., Stoltzfus, C.M. & Sonza, S. (2008) “HIV-1 infection induces changes in expression of cellular splicing factors that regulate alternative viral splicing and virus production in macrophages” Retrovirol. 5, 18 (http://www.retrovirology.com/content/5/1/18)

                       

172. Fulcher, A.J., Ahmed, C.M.I., Noon-Song, E.N., Kwan, R.Y.Q., Subramaniam, P.S., Johnson, H.M. & Jans, D.A. (2008) “Interferon g is recognised by importin a/b: enhanced nuclear localising and transactivation activities of an interferon g mimetic” FEBS Lett. 582, 1569-1574

                       

173. Li, D-S., Jans, D.A., Bardin, P.G., Meanger, J., Mills, J. & Ghildyal, R. (2008) “Association of respiratory syncytial virus M protein with viral nucleocapsids is mediated by the M2-1 protein” J. Virol. 82, 8863-8870

 

174. Caly, L., Nishimura, S., Jans, D.A. & Piller, S.C. (2008) “Cleavage at novel sites within virions and transfected cells of GFP-HIV-1 Vpr fusions: concerns for intracellular trafficking studies” J. Fluor. 19, 567-73

 

175. Caly, L., Saksena, N.K., Piller, S.C. & Jans, D.A. (2008) “Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor” Retrovirol. 5 67 (http://www.retrovirology.com/content/5/1/67)

 

176. Kuusisto, H., Wagstaff, K.M, Alvisi, G. & Jans, D.A. (2008) “The C-terminus of apoptin represents a unique tumor cell-enhanced nuclear targeting module” Int. J. Cancer 123, 2965-2569

 

177. Sinigalia, E., Alvisi, G., Mercorelli, B., Coen, D.M., Pari, G.S., Jans, D.A., Ripalti, A., Palù, G. & Loregian, A. (2008) “Role of homodimerization of human cytomegalovirus DNA polymerase accessory protein UL44 in origin-dependent DNA replication in cells” J. Virol. 82, 12574-12579

 

178. Rawlinson, S.M., Pryor, M.J., Wright, P.J., & Jans, D.A. (2009) “CRM1-mediated nuclear export of dengue virus NS5 RNA polymerase regulates the kinetics of virus production” J. Biol. Chem. 284, 15589-15603

 

179. Ghildyal, R., Ho, A., Dias, M., Soegiyono, L., Bardin, P.G., Tran, K.C., Teng, M.N. & Jans, D.A. (2009) “The Respiratory Syncytial Virus Matrix Protein Possesses A Crm1-Mediated Nuclear Export Mechanism” J. Virol. 83, 5353-5362

 

180. Ghildyal, R., Jordan, B., Li, D., Bardin, P.G., Gern, J.E. & Jans, D.A. (2009) “Rhinovirus 3C protease can localize in the nucleus and alter active and passive nucleocytoplasmic transport” J. Virol. 83, 7349-7352

 

181. Glover, D.J, Ng, S.M., Mechler, A., Martin, L.L. & Jans, D.A. (2009) “Multifunctional Protein Nanocarriers for Targeted Nuclear Gene Delivery in Non-Dividing Cells” FASEB J. 23, 2996-3006 233    [Featured on Front Cover and in press release of Federation of American Societies for Experimental Biology. Media Release 31.6.2009 “Finding the ZIP-code for gene therapy: Scientists imitate viruses to deliver therapeutic genes”]

 

182. Itman, C., Small, C., Griswold, M., Nagaraja, A., Matzuk, M., Brown, C., Jans, D.A. & Loveland, K.L. (2009) “Developmentally regulated SMAD2 and SMAD3 utilization directs activin signalling outcomes.” Dev. Dynamics 238, 1688-1700

 

183. Foong, Y.F., Jans, D.A., Rolph, M.S., Gahan, M.E. & Mahalingam, S. (2009) “Interleukin-15 mediates potent antiviral responses via an interferon-dependent mechanism.” Virol., in press July 15 (VIRO-09-221R1)

   

184. Roth, D.M., Harper, I., Pouton, C.W. & Jans, D.A. “Modulation of nucleocytoplasmic trafficking by retention in cytoplasm or nucleus” J. Cell Biochem. 107, 1160-1167

 

185. Alvisi, G., Roth, D.M., Camozzi, D., Pari, G.S., Loregian, A., Ripalti, A. & Jans, D.A. (2009) “The flexible loop of human cytomegalovirus DNA polymerase processivity factor ppUL44 is required for efficient DNA binding and replication in cells” J. Virol. 83, 9567-9576

 

186. Moseley, G.W., Lahaye, X., Roth, D.M., Oksayan, S., Filmer, R.P., Rowe, C.L., Blondel, D. & Jans, D.A. (2009) “Dual modes of rabies P-protein association with microtubules: a novel strategy to suppress the anti-viral response” J. Cell Sci., in press (ID#: JOCES/2008/045542)

 

187. Sivakumaran, H., van der Horst, A., Fulcher, A.J., Apolloni, A., Lin, M-H., Jans, D.A., & Harrich, D. (2009) “Arginine Methylation Increases the Stability of HIV-1 Tat” J. Virol., in press (JVI00499-09  R1)

 

188. Glover, D.J., Leyton, D. L., Moseley, G.W. & Jans, D.A.,  (2009) "The efficiency of nuclear plasmid DNA delivery is a critical determinant of transgene expression at the single cell level" J. Gene Med., in press (JGM-09-0091.R1)