Dr Georg Ramm
Senior Research Fellow
Office Room CG23 / Building 13C
Lab Room D231 / Building 13D
MSc (Diplom), Biochemistry, 1995, Max-Planck-Research Unit, Halle, Germany
PhD, Cell Biology, 2000, Utrecht University, The Netherlands
Membrane Biology GroupThe membrane biology group is performing research into the regulation of membrane trafficking. Our emphasis is on the spatial organisation of signalling cascades that control membrane trafficking events. Our group has strong expertise in electron microscopy techniques which are used in combination with biochemical and molecular biological techniques and advanced fluorescence imaging.
Regulatory Mechanisms of Autophagy
Autophagy delivers cytoplasmic proteins and organelles to the lysosome for degradation and is upregulated during starvation to free up extra energy for survival, but is also needed to degrade malfunctioning organelles. The Serine/Threonine kinase ULK1 (Unc-51 like kinase) is emerging as a key regulatory kinase in autophagy and we aim to better understand its regulation. A focus is on the spatial organisation of autophagic signalling events and to understand its role in autophagy regulation.
Membrane Morphology and Disease
Altered membrane turnover and morphology is associated with a wide spectrum of diseases. In collaboration we perform morphological research into aspects of cancer, diabetes, infectious and other diseases.
Integration of Optical and Electron Microscopy
Optical and electron microscopy exhibit different strength. Optical microscopy can reveal the relative position of fluorescently labelled molecules in live cells. On the other hand electron microscopy can visualise complicated cellular ultrastructures. Approaches that combine the advantages of light microscopy with those of electron microscopy can reveal unique insights. A focus of the work in our electron microscopy lab is on the establishment and application of correlated light and electron microscopy (CLEM) approaches.
Ben Padman, PhD student
Aaron Alfred Gray, student
Bio Electron Microscopy Facility
Joan Clark, Professional Officer
Viola Oorschot, Professional Officer
Adam Costin, Technical Officer
Padman, B.S., Ramm, G. (2014). Live-Cell CLEM of Subcellular Targets: An Optimized Procedure for Polymer-Based Imaging Substrates. Methods in Cell Biology 124 pp.275-304.
Oorschot, V.M.J., Sztal, T.E., Bryson-Richardson, R.J., Ramm, G. (2014). Immunocorrelative Light and Electron Microscopy on Tokuyasu Cryosections. Methods in Cell Biology 124 pp.241-257.
Padman, B.S., Bach, M., Ramm, G. (2014). An Improved Procedure for Subcellular Spatial Alignment during Live-Cell CLEM. PLoS One 9(4):e95967.
Padman, B.S., Bach, M., Lucarelli, G., Prescott, M., Ramm, G. (2013). The protonophore CCCP interferes with lysosomal degradation of autophagic cargo in yeast and mammalian cells. Autophagy 9 (11) pp.1862-75.
Tan, S.X., Ng, Y., Burchfield, J.G., Ramm, G., Lambright, D.G., Stöckli, J., James, D.E. (2012). The Rab GTPase-activating protein TBC1D4/AS160 contains an atypical phosphotyrosine-binding domain that interacts with plasma membrane phospholipids to facilitate GLUT4 trafficking in adipocytes. Molecular and Cellular Biology 32 pp. 4946-4959.
Klionsky, D.J; Abdalla, F.C.; ...; Ramm, G.; ...; Zuckerbraun, B. (2012). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8, pp.1-100.
Bach, M., Larance, M., James, D., Ramm, G. 2011, The Serine/Threonine Kinase ULK1 is a target of multiple phosphorylation events, Biochemical Journal, 440: pp. 283-291.
Bach, M., Ramm, G. 2011, How to control self-eating habits: Metabolic control of autophagy, Australian Biochemist. 42: pp. 17-20.
Ng, Y., Ramm, G., and James, D.E. Dissecting the mechanism of insulin resistance using a novel heterodimerization strategy to activate AKT. J Biol Chem. 2009 Dec 18. [Epub ahead of print]
Ng, Y., Ramm, G., Burchfield, J.G., Coster, A.C., Stöckli, J., and James D.E. Cluster analysis of insulin action in adipocytes reveals a key role for Akt at the plasma membrane. J Biol Chem. 2009 Nov 6. [Epub ahead of print]
Stöckli, J., Davey, J.R., Hohnen-Behrens, C., Xu, A., James, D.E., and Ramm, G.# (2008) Regulation of GLUT4 Translocation by the RabGAP AS160/TBC1D4. Role of Phosphorylation and Membrane Association. Mol. Endocrinol. 22: 2703-15. IF 5.33
Yip, F.M.F., Ramm, G., Larance, M., Wagner, M.C., Guilhaus, M., and James, D.E. (2008) Phosphorylation of the Myosin Motor Myo1c Is Required For Insulin-Stimulated GLUT4 Translocation in Adipocytes. Cell Metab 8, 384-98. (Cover story & Preview Cell Metab 8, 344-6). IF17.14
Ng, Y., Ramm, G., Lopez, J.A., and James, D.E. (2008) Rapid activation of Akt2 is sufficient to stimulate GLUT4 translocation in 3T3-L1 adipocytes. Cell Metab 7, 348-56. IF 17.14
Larance, M., Ramm, G. and James, D.E. The GLUT4 code. (2008) Mol. Endocrinol. 22, 226-33. IF 5.33
Ramm, G.#, Larance, M., Guilhaus, M. and James, D.E. # (2006). A role for 14-3-3 in insulin-stimulated GLUT4 translocation through its interaction with the RabGAP AS160. J Biol Chem 281, 29174-29180. # joint corresponding author. IF 5.58
Larance, M.*, Ramm, G.*, Stöckli, J.*, van Dam, E. M., Winata, S., Wasinger, V., Simpson, F., Graham, M., Junutula, J. R., Guilhaus, M., and James, D. E. (2005). Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking. J Biol Chem 280, 37803-37813. *joint first authors. IF 5.58
Ramm, G., and James, D. E. (2005). GLUT4 trafficking in a test tube. Cell Metab 2, 150-152. IF 17.14
Kleijmeer, M.*, Ramm, G.*, Schuurhuis, D., Griffith, J., Rescigno, M., Ricciardi-Castagnoli, P., Rudensky, A. Y., Ossendorp, F., Melief, C. J., Stoorvogel, W., and Geuze, H. J. (2001). Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol 155, 53-63. (Cover story) *joint first authors. IF 9.6. F1000: exceptional article.
Ramm, G., Slot, J. W., James, D. E., and Stoorvogel, W. (2000). Insulin recruits GLUT4 from specialized VAMP2-carrying vesicles as well as from the dynamic endosomal/trans-Golgi network in rat adipocytes. Mol Biol Cell 11, 4079-4091. IF 6.03