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Project Planning

  • The Facility is accessible to any academic investigators from any institution as well as industrial clients.
  • The facility can be accessed by initial contact with either Mrs Shane Reeve or Dr David Steer at shane.reeve@med.monash.edu.au or david.steer@med.monash.edu.au or by phone +613 9905 3779 fax +613 9905 3726.
  • Service can cover anything from a simple one off analysis to an ongoing collaborative project in which case a meeting will be arranged to discuss the most appropriate way forward.
  • Discussion will include:
    • The objectives of the study.
    • The information already known about the particular protein or protein extract including physical properties such as hydrophobicity, pI or MW.
    • The amount of material available for the study.
    • The anticipated relative abundance of the particular proteins of interest.
    • The extent of available genome and/or protein sequence information of the organism under investigation.
    • Any budgetary or time constraints for the project.
    • The possibility for the collaborator to be given appropriate access and training to use the lab facilities.
  • Samples are to be submitted with the service request form
  • Neurosciences related samples should be submitted via the NSV proteomics and genomics platform www.nnf.com.au/genprot or by direct contact with Dr. David Steer
  • We currently offer the following services:
    • Gel spot/band excision.
    • 1D and 2D gel electrophoresis.
    • Sensitive imaging of gels stained with Coomassie and silver stains.
    • Software to enable 2D gel based expression analysis.
    • Enzymatic digests including deglycosylation.
    • Protein identification from 2D gel spots and 1D gel bands.
    • N-terminal sequencing from blots, gel bands and purified samples.
    • Analysis by mass spectrometry of peptides/proteins including molecular weight determination and sequencing.
    • Equipment in the lab; Applied Biosystems 4700 MALDI-TOF-TOF including Mascot data base, Agilent 1100 series HPLC, Applied Biosystems Procise protein analyzer, Ettan DALT 12 24cm gel rig, Hoefer mini VE 7cm gel rig, Ettan IPGphor IIb