| Contacts |
Histology Platform ManagerCamilla Cohen Phone +61 3 990 52740 Mobile +61 407 810274 Email: camilla.cohen@monash.edu Histology Paraffin LaboratoryPhone: +61 (03) 99052740/43 Email: histology@monash.edu Histology Resin LaboratoryPhone: +61 (03) 99052708 Email: histology@monash.edu
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Everything we do is focussed on assisting you, in all aspects of Histology so you can engage in what you do best.
Your partner in research and education-
World class, full service histology laboratory
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Consultation and collaboration
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Paraffin, frozen section and specialist staining services
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Expert training in all histological techniques
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Specialist methacrylate and epoxy resin methods
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Immunohistochemical techniques
The Histology Platform has two service streams; a full end-to-end service provided by specialist staff, or a “Do it Yourself” service providing access to laboratory facilities, specialist equipment and consumables. In addition, the experienced Histology team can provide advice, training and research assistance. The Histology Platform operates on a fee for services basis for all services and laboratory access.
Two laboratories operate within the Histology Platform. They have been developed to meet the quality and high throughput needs of researchers, academic staff and students.
The paraffin and resin laboratories are equipped with a range of state-of-the-art equipment including:
Fully equipped dissection area and cassette writer
Rapid, dual processing Peloris tissue processor providing efficient, reliable and timely processing to paraffin wax
Embedding units for high quality paraffin blocking
Modern microtomes for specimen sectioning
Dake Autostainer Plus with PT link for antigen retrieval for all Immunohistochemical staining requirements
Leica Ultra microtomes for methacrylate and epoxy resins for light and electron microscopy
Stereomicroscope
Fully equipped staining area including an automated staining unit with coverslipper, together with manual options for DIY staining and coverslipping
Cryostat for frozen sectioning
MTA Tissue Arrayer for multiple core block generation
Aperio Scanscope for digital scanning of microscopy slides - coming soon, due August 2012
Other specialised equipment such as a sledge microtome, digital camera for digital microscopy and multiheader microscope
Hours of operation:
Paraffin laboratory:
8.30am-5.00pm Monday-Thursday,
9.00am-5.00pm Friday
Resin laboratory:
9.00am-4.30pm Monday - Thursday,
9.00am-3.00pm Friday
Staff:
Dr. Ian Smyth
Histology Platform Scientific Director, ARC Research Fellow in the Department of Anatomy and Developmental Biology and Department of Biochemistry and Molecular Biology. Interested in the development of diseases affecting skin and kidney. He has 15 years extensive experience in studying animal models of disease. Contact: Ian.Smyth@monash.edu
Mrs Camilla Cohen
Histology Platform Manager has worked in the Histology field for over 25 years in both hospital and private clinical histopathology laboratories. Has extensive experience in routine histology, laboratory quality assurance and management. An accredited NATA assessor and skilled in Immunohistochemical techniques and frozen sections.
Mrs Stefania Tombs
Laboratory Technician has worked in the field for 25 years. Experienced in both research and diagnostic histopathology laboratories. Skills include enzyme histochemistry, immunohistochemistry and frozen sectioning. Specialist in muscular disorders.
Mrs Sue Connell
Laboratory Scientist has worked in resin histology and electron microscopy for over 30 years. Trained in electron microscopy on site and in conjunction with the Alfred Hospital. Resident expert in resin section cutting for both light and electron microscopy.
Mrs Julie Hickey
Laboratory Technician has over 20 years’ experience in resin histology and electron microscopy. Has worked exclusively in research at Melbourne and Monash University. In house resin staining specialist experienced in routine and special stains.
Histology charges
All personnel utilising Monash Histology Platform services are required to pre-register as an Approved User. Registration is required for all services offered, OH&S regulations, and certification of payments by Monash Finance. Enquiries regarding registration fees can be directed to the Histology Manager.
Histology Platform Do It Yourself
Paraffin
| Processing/Embedding: | $6.50 per block |
| Sectioning: | $10.00 per hour for use of microtome |
| Cryostat sectioning: | $20.00 per hour |
Staining on paraffin/frozen sections:
| Haematoxylin & Eosin | $1.65/slide |
| Periodic acid/Schiffs (PAS) | $2.75/slide |
| Masson Trichrome | $3.30/slide |
| Luxol Fast Blue/Cresyl Violet | $3.50/slide |
| Luxol Fast Blue/PAS | $4.00/slide |
| Alcian Blue | $3.00/slide |
| Elastin (Hart/s/Weigerts) | $6.50/slide |
| Gordon & Sweets (silver stain) | $6.50/slide |
| Oil Red O | $3.00/slide |
| Perls Prussian Blue | $4.00/slide |
Miscellaneous charges:
| Dewaxing/coverslipping slides only | $1.00/slide |
| Dewaxing/staining with Haematoxylin only | $1.50/slide |
Other specialised stains/methods are available, prices on request.
Resin
Processing:
| Prices on enquiry (Sample dependant) |
Sectioning:
| $15 per hr (Anatomy users) |
| $20 per hr (all other users) |
Staining Glycolmethacrylate (resin):
| Haematoxylin & Eosin: | $1.50 per slide |
| PAS | $2.50 per slide |
Histology Platform Professional Services
Paraffin
| Histology (Paraffin) Services: | $70.00 per hour |
| Processing/Embedding: | as for DIY charges, plus hourly rate |
| Cutting (paraffin or cryostat): | Microscope slide rate per slide ($1.50), plus hourly rate |
| Staining: | As for DIY staining, plus hourly rate |
| Technical Assistance/training/teaching/problem solving: | $70.00 per hour |
Resin
Processing:
| Prices on enquiry (Sample dependant) |
Cutting:
| $45 per hr (Anatomy users) |
| $70 per hr (all other users) |
Staining:
| As for DIY charges plus hourly rate |
Technical Assistance/training/teaching/problem solving:
| $45 per hr (Anatomy users) |
| $70 per hr (all other users) |
Consumables Paraffin and Resin:
Prices on enquiry
